Strawberry DNA

Today I was testing out a protocol for isolating DNA from a strawberry for class tomorrow. It worked beautifully. That white slimy stuff on the stick is the DNA. As well as the bubbly wispy strands floating in the clear portion of the solution. Materials used: strawberries, dishwashing liquid, water, salt and cold 95% ethanol (well, -20C actually).

Protocol taken from The Tech Museum

Each strawberry yields around 25 mls of strawberry extract- enough for 25 students.

For 3 strawberries:
~1/2 tsp salt (2.5 g)
~1/3 cup H2O (80 ml)
~1 tbs liquid detergent (15 ml)
~Chill ethanol (rubbing alc should be fine; no need to break out the Everclear) in freezer

1. Mix salt, water, and detergent together in a bowl to make the extraction liquid. This is double what you need in case you mess up. (~1 tbsp/strawberry).
2. Squish strawberries in a plastic bag.
3. Add 3 tbsp (45 ml) extraction liquid. Squish thoroughly.
4. Strain through a cheesecloth.
5. Aliquot ~1ml, or more, into each tube.
6. Slowly add cold ethanol on top. DNA precipitates at the interface.

25 Random Jules Things

1. I’m a procrastinator at heart. It’s a tough battle.
2. I can become very fixated on food when I’m hungry. Don’t get in my way.
3. I’ve never met a food type that I wouldn’t at least try once.
4. I’ve been playing violin for 17 years.
5. I started off playing piano when I was 8 and absolutely hated it. Then I switched to violin and was happier.
6. I have eight piercings in my ears. I don’t wear earrings much anymore.
7. I have contacts, but I only wear them on weekends.
8. My hair has been completely blue, purple and red at different points in my life.
9. I kill plants for a living. I like plants.
10. I’ve never owned a pet.
11. I’ve only ridden on a motorcycle once, and it wasn’t with mike.
12. I lived in the Berkeley Co-op system for the entire duration of my undergraduate career.
13. I’ve played at Davies Symphony Hall and in Miami with my community orchestra.
14. In high school, I’ve toured the eastern coast of Australia and New Zealand for a month, playing with a philharmonic group in portland. It was amazing.
15. I like to follow food blogs.
16. I know random weird things.
17. I have a habit of running people I know off the sidewalk. Their gravity trumps mine.
18. I enjoy minimalism, and like occasional pops of bright colors.
19. I don’t have a favorite cuisine.
20. In elementary school, I messed up in a spelling bee. The word was “balloon”.
21. I don’t like clowns.
22. I prefer walking to bicycling
23. I don’t like to drive, but will if I have to.
24. I once got lost in SF, pulled my car into a parking garage, and called for help. I was one block away from my destination.
25. Vanilla ice cream serves as a vehicle for my crushed pistachios.

Cheesecake

This week, I got it in my head that I wanted to make cheesecake. I didn’t remember what recipe I used before, but this time, I followed Alton Brown’s Sour Cream Cheesecake recipe . The recipe calls for the use of a 9 inch cake pan. I had 4-4.5 inch springform pans. So what was I to do? Not only was I trying a new recipe, I was also attempting to modify it! And I don’t own an electric mixer. But chopsticks and spatulas have not failed me yet, so I was good to go.

While I stayed fairly true to ingredient amounts, I modified the oven time to account for my individual pans and reviewer comments on how the bake times were too short. I paced about nervously like a soccer mom with too much caffeine. I would turn on the oven lights every ten minutes or so, and violently shake the oven so I could see how much the cheesecake jiggled- much like what people do to the vending machines that refuse to relinquish their product. And the verdict always was: Still jiggly! Curses!

Back to more pacing for ten minutes before I would check again. Mike thought I was a bit loopy. The cheesecake was still very jiggly after 1.5 hours. I gave up and found that it set nicely after I turned off the oven. Whew. The end result turned out quite well.

AB Cheesecake Recipe, modified to produce four smaller cheesecakes:

Crust:
* 1 sleeve plus a few Honeymaid graham crackers
* 3/4 unsalted butter (3 oz) plus a lil
* 1 tablespoon sugar

Filling:
* 20 ounces cream cheese
* 1 1/4 cups sour cream
* 1 cup sugar
* 1 tablespoon vanilla extract
* 2 eggs
* 3 yolks
* 1/3 cup heavy cream

Directions

Preheat oven to 300 degrees F. Line individual pans with foil, and brush with butter. Fill two large pyrex pans with hot hot water (boiling would be better, but I just turned the tap to the hottest possible setting because I’m lazy) and place in oven to preheat.

Combine crumbled graham crackers, melted butter, and 1 tablespoon of sugar. Press mixture into the bottom of each pan. Place remaining crumbs on foil and bake both the crust and the remaining mixture for 10 minutes. Cool. Reserve additional crumb mixture for sides.

Beat sour cream for 10 seconds. Add the cream cheese and cup of sugar and mix for 30 seconds or so. More if you have lumps.

In a separate container, combine vanilla, eggs/yolks and heavy cream in a small bowl. Slowly pour this mixture into the cream cheese mixture while stirring with a spatula. Once completely combined, pour into cooled crusts.

Lower oven temperature to 275 degrees F. Place cheesecakes into the preheated water baths in the oven for 1.5 hours. Open the oven door for a little bit (10 seconds). Turn the oven off, close door and leave cheesecake to set for one more hour. Remove the cheesecake from the water bath and place in the refrigerator to completely cool. ( I admit that Mike and I ate one right away. It was 11pm, and we wanted cheesecake! :D )

When ready to serve, take the remaining graham cracker mixture and press into the sides of the cake.

The Poisoned Toothpick

I inoculated some media with bacteria to grow overnight so I could make some stocks. When I came back the next morning, I noticed something odd. The culture on the right should look just like the culture on the left. Instead, it looks purple, and the cells don’t look very happy! Apparently, sometimes autoclaved toothpicks can cause this. My poor bacteria got the short end of the stick. Poor unhappy bacteria T_T In the  6 years that I’ve used toothpicks  to inoculate media, this is the first time that I’ve seen this. I guess I should use a different method from now on. Just in case. How curious.

My Favorite Snack

The snack (well, mini lunch, really) that I tend to make most often involves slices of bread, fruit, and cheese. While the cheese and fruit are highly variable, the bread is not substitutable. Whole Grains Bakery makes a dark russian rye bread that is full of pumpernickel and just the right amount of caraway seed. This bread toasts nicely, and each bite is earthy and flavorful. MmMMMmmm… pumpernickel…

and now it is all in my belly. Mwahahah… okay, mike had some too.. that thief…

Mother of Thousands

So I acquired a baby  plantelet from Kalenchoe (Mother of Thousands) from one of my classmates several months ago. It’s a cool plant that they often use for tumor research. I used to have one in undergrad. It’s currently growing on the window sill right next to my desk in lab. It is now producing little plantelets along the leaf margins. Soon they will ring each leaf, with little dangling roots. These will soon drop off into the soil to sprout up into new Kalenchoe clones. Woot. It’s pretty nifty.

What Does Your Name Mean?

I’ve been slacking on improving my chinese, but I’m determined to get back to that. Today, mike showed me that my macbook had a built in ability to type chinese. Woot. I’ll have to show it to my parents. In the mean time, I used my trusty resources to piece together what Mike’s name means. I used it to figure out the cantonese pronunciation as well, since he always pronounces it in mandarin. Cuz I’m biased like that. I’m pretty happy that I finally got around to figuring out how to pronounce his name though… hehe.. now I can use his chinese name when I’m mad. Right? Right?

The characters in parantheses signify verb, adjective and noun forms, respectively. Cantonese pronunciation is bracketed; mandarin pronunciation is not.  So Mike is a vibrating honor filled person? Or maybe he restores honor and glory? Hmmm.. what a bright and shiny name.

PMB Halloween Party

So we had our dept Halloween Party today, and there was a costume contest. 3 of us decided to be a “Ferrous Wheel” because we though it would be very punny (ehehe) with our Fe2+ (ferrous iron). So half an hour before the party, we made big cardboard raindrops with Fe2+ written in glitter glue, and when our turn came about, we linked arms and spun round and round to be the “wheel”. People seemed to enjoy our joke, and we almost won. The group that beat us out was a production put on by this entire lab. They were all parts of a gigantic magnetotactic bacteria. They obviously put in way more effort than us. So I was surprised by how much applause we got. I think they liked that we danced around in a circle. But next year… we shall totally own them.

Bamboo Fun

I bought a little Wacom Bamboo Fun tablet to play around on. It’s a little awkward to use right now, but I’m sure it will get more comfortable with time. And hopefully my drawing skills will improve as well. Time to get back to work!